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Vaporized Marijuana illegal?
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Posted 2009-05-01, 01:09 PM in reply to
Sum Yung Guy
's post starting
"Your silly... carrying around a bag of..."
Biochem 101: How to design a Cannabis-equivalent citrus plant
* Step One:
Biochemically isolate all the required enzymes for the production of THC.
* Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
* Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
* Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.
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